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FeTMPyP increases the reactive oxygen species (ROS) levels in the isolated brain mitochondria. Mitochondria were isolated from the wild-type (WT) mice brains and incubated with peroxynitrite (PN) (5 µmol/L), SIN-1 (50 µmol/L), and FeTMPyP (2.5 µmol/L) for 30 min (for ROS, n = 8–10 mice). ROS levels were measured using dihydroethidium (excitation: 510 nm and emission: 600 nm). Data are presented as means ± SE and analyzed by one-way ANOVA. *P ≤ 0.05, statistically significant.
