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. 2020 Dec 18;320(2):H772–H786. doi: 10.1152/ajpheart.00606.2020

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Published by the American Physiological Society.

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Figure 8. — Quantitative analysis by real-time PCR showing the effects of hypothalamic paraventricular nucleus (PVN) microinjection of tumor necrosis factor (TNF)-α (25 ng/side) on the mRNA expression of (A) angiotensin-converting enzyme (ACE), (B) angiotensin II type-1a receptor (AT_1a-R), (C) cyclooxygenase (COX)-2, (D) NF-κB p65 subunit, and NADPH oxidase subunits (E) p47^phox and (F) gp91^phox in the cortex, subfornical organ (SFO), and PVN in rats pretreated with PVN vehicle (VEH) or AG1478. Values are mean ± SE (n = 6 for each group) and expressed as a fold change relative to VEH control. *P < 0.05 compared with the PVN VEH. †P < 0.05, PVN AG1478 + PVN TNF-α vs. PVN VEH + PVN TNF-α.