Table 1.
Sequences for primers
| Gene | Sequences |
|---|---|
| ACE | |
| Forward primer:Reverse primer: | 5′-GTGTTGTGGAACGAATACGC-3′5′-CCTTCTTTATGATCCGCTTGA-3′ |
| AT1aR | |
| Forward primer:Reverse primer: | 5′-ACTCACAGCAACCCTCCAAG-3′5′-ATCACCACCAAGCTGTTTCC-3′ |
| COX-2 | |
| Forward primer:Reverse primer: | 5′-GGCACAAATATGATGTTCGCA-3′5′-CCTCGCTTCTGATCTGTCTTGA-3′ |
| NF-κB p65 | |
| Forward primer:Reverse primer: | 5′-GCATCCACAGCTTCCAGAAC-3′5′-GCACGGTTATCAAAAATCGG-3′ |
| p47phox | |
| Forward primer:Reverse primer: | 5′-AGGTTGGGTCCCTGCATCCTATTT-3′5′-TGGTTACATACGGTTCACCTGCGT-3′ |
| gp91phox | |
| Forward primer:Reverse primer: | 5′-CGGAATCTCCTCTCCTTCCT-3′5′-GCATTCACACACCACTCCAC-3′ |
| GAPDH | |
| Forward primer:Reverse primer: | 5′-AAGGTCATCCCAGAGCTGAA-3′5′-ATGTAGGCCATGAGGTCCAC-3′ |
ACE, angiotensin-converting enzyme; AT1aR, angiotensin II type 1a receptor; COX-2, cyclooxygenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NF-κB p65, nuclear factor kappa B p65 subunit; p47phox, gp91phox, NADPH oxidase subunits.