Fig. 3.

Decreased fertility and premature ovarian failure in SHR females carrying Prdm9 deletions. a, b Boxplots of ovary weight (a, in mg) and of relative ovary weight (b, mg ovary weight per gram of body weight). c Examples of HE stained sections from three adult rat ovaries at two magnifications. Note that all females carry germ cell follicles (arrows). d Litter size of female parents of two indicated Prdm9 genotypes with maternal age at birth. The difference between genotypes was tested using the LRM in the age group of up to 150 dpp. e Number of pups per month per female (OMU) and mating ranges (days) for two indicated Prdm9 genotypes. The abscissa at OMU = 0 contains data for 14 Prdm9-deficient females, ten of which were mated after 150 dpp (for additional mean of 26 days). f Follicle quantification from ovarian sections of the indicated Prdm9 genotypes at three points of postnatal development. Differences between three Prdm9 genotypes and between animals of three ages within each genotype were analyzed using LRM with subsequent Tukey test and Bonferroni adjustment. g Representative images of HE-stained ovarian sections from 21-dpp females