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. 2021 Apr 29;13:50. doi: 10.1186/s13098-021-00664-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Hesperidin upregulated miR-149 expression by inhibiting DNMT1. a The effect of hesperidin on the expression of miR-149 was detected by qRT-PCR. b The CpG site of miR-149 in the promoter region (1000 to − 100 bp) was analysed by MethPrimer software (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi). c The effect of hesperidin on the methylation level of the miR-149 promoter was analysed by MSP assay. d The effect of hesperidin on the protein level of DNMT1 was determined by western blotting. e Transfection efficiency of the siDNMT1 plasmids was detected by qRT-PCR. f The effect of DNMT1 knockdown on the expression of miR-149 was detected by qRT-PCR. All the data were presented as the mean ± S.D. *p < 0.05, **p < 0.01, *** p < 0.001