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. 2021 Apr 29;22:120. doi: 10.1186/s13059-021-02336-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Evidence of heteroplasmy associated with a tandem repeat in the kakapo mitochondrial genome. a Fraction of CLR reads that support the copy number in the MitoVGP reference (blue bar is 11 copies). b IGV [54] plot showing the PacBio CLR alignment of reads that fully span the ~ 925-bp-long tandem repeat (between green dashed lines, repeat unit = 84 bp), highlighting the presence of reads that support the copy number of 11 in the mitoVGP reference, but also reads supporting fewer copies of the repeat (red arrows, black in panel a)