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. Author manuscript; available in PMC: 2021 Apr 29.
Published in final edited form as: J Med Chem. 2020 Aug 28;63(17):9838–9855. doi: 10.1021/acs.jmedchem.0c01016

Figure 4.

Figure 4.

Common genes/proteins between Bru-seq and proteomics analysis reveal that QD394 interferes with mitochondrial gene expression and RNA catabolic process. (A) Venn diagrams of upregulated and downregulated genes/proteins between Bru-seq (fold change over 2) and proteomics (fold change over 1.5) analysis. (B) STRING analysis of 35 downregulated genes/proteins in common between Bru-seq and proteomics studies. Red circle highlights the genes involved in mitochondrial gene and translation regulation. Blue circle indicates the genes associated with the oxidation reduction process. (C) LRPPRC and PNPT1 are significantly related to the overall survival of pancreatic adenocarcinoma patients in TCGA dataset. (D) QD394 decreased the protein expression of LRPPRC and PNPT1 in MIA PaCa-2 cells. QD394 and napabucasin were tested at 3 × IC50 for 1 h and 4 h. QD394 was tested at 2 × IC50 for 24 h. H2O2 was used at 150 μM. * denotes p < 0.05, ** denotes p < 0.005, *** denotes p < 0.0005. (E) The expression levels of mitochondrial proteins decreased in the QD394-treated MIA PaCa-2 cells.