Table 4.
A group of web-based algorithm for gRNA designing
| Tool name | Off-target analysis | Webpage link |
|---|---|---|
| CRISPR Design Tool | Yes | https://www.synthego.com/products/bioinformatics/crispr-design-tool/ |
| Cas-Designer | No | http://www.rgenome.net/cas-designer/ |
| CRISPR MultiTargeter | No | http://www.multicrispr.net/ |
| CRISPR Genome Analysis Tool | http://cbc.gdcb.iastate.edu/cgat/ | |
| GeneArt | Yes | https://www.thermofisher.com/sa/en/home/life-science/genome-editing/geneart-crispr/geneart-crispr-search-and-design-tool.html/ |
| ZiFiT Targeter Version 4.2 | No | http://zifit.partners.org/ZiFiT/ChoiceMenu.aspx/ |
| CRISPR/Cas9 target online predictor | Yes | https://crispr.cos.uni-heidelberg.de/ |
| Cas9 online designer | Yes | http://www.rgenome.net/cas-designer/ |
| CHOPCHOP | Yes | http://chopchop.cbu.uib.no/ |
| sgRNAcas9 | Yes | http://www.biootools.com/col.jsp?id=103/ |
| E-CRISP | Yes | http://www.e-crisp.org/E-CRISP/ |