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. 2021 Apr 15;11:611544. doi: 10.3389/fonc.2021.611544

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Copyright © 2021 Liu, Gao, Chen, Zhao, Sun, Zou, Guan, Yang, Pei, Wang, Wang, Li and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of WTAP inhibits the proliferation and invasion of in vitro HCC cells. (A) SMMC-7721 or Hep3B cells were transfected with different short hairpin RNA (shRNAs) targeting WTAP or control shRNA. WTAP protein expression was analyzed by a western blot 48 h later. GAPDH was used as a loading control. Hep3B or SMMC-7721 cells were transfected with sh-WTAP or control shRNA. (B–D) Effects of the knockdown of WTAP on the cell proliferation were determined by a cell counting kit-8 (CCK-8) assay (B), a colony formation assay (C), and the 5-Ethynyl-2′-deoxyuridine (EdU) staining assay (D). Data were represented as the mean ± SD. (E,F) The invasion and migration abilities of HCC cells were assessed by using the Transwell invasion (E) and wound healing assays (F). *p < 0.05, **p < 0.01.