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. 2021 Apr 15;11:611544. doi: 10.3389/fonc.2021.611544

Figure 6.

Figure 6

WTAP is a direct binding target of miR-139-5p. (A) Predicted target site of miR-139-5p on the 3′ untranslated region (3'-UTR) of WTAP and the mutated WTAP 3′-UTR sequences. (B) Quantification of the miR-139-5p expression levels in HCC and normal tissues through quantitative PCR (qPCR) (n = 22). (C) HEK293 cells were transfected with a reporter vector containing wild type (wt) or mutant (mut) 3′-UTR of WTAP, together with miR-139-5p mimics or negative control. The relative luciferase activity was assessed for 48 h later. The mRNA (D,E) and protein (F) levels of WTAP in Hep3B or SMMC-7721 cells transfected with miR-139-5p mimics, inhibitor, and negative control were analyzed by qPCR and western blot. (G) Pearson correlation analysis of the relationship between the WTAP expression and miR-139-5p expression in HCC tissues. *p < 0.05, **p < 0.01.