Figure 7.

Overexpression of miR-139-5p inhibits the cell proliferation and invasion by targeting WTAP. Hep3B or SMMC-7721 cells were transfected with NC, miR-139-5p mimics or miR-139-5p mimics, and the WTAP overexpression plasmid. (A) The expression levels of E-cadherin, N-cadherin, Slug, Snail, and β-catenin were analyzed by western blot 48 h later. GAPDH was used as a loading control. (B,C) Cell proliferation was determined by the CCK-8 cell proliferation assay (B) and the colony formation assay (C). (D) The cell invasion capability of Hep3B or SMMC-7721 cells in the different groups was analyzed by the Transwell assay. *p < 0.05, **p < 0.01.