Figure 2.

Imaged analysis to quantify the magnification ratio of the intravital retinal imaging of mice at different ages. (a) Representative intravital imaging of the retinal vasculature in mice with different ages of 3 and 22 weeks, showing a decreased refractive power of the mouse eye with growth. Yellow circles delineate a similar-sized area. (b) Representative images of the same retinal vasculature to calculate the magnification ratio between the in vivo and ex vivo imaging at different ages. Scale bars: 300 µm in vivo and 100 µm ex vivo. (c, d) Magnification ratio at different ages of the mice strains of (c) C57BL/6 and (d) Balb/c, respectively. (e, f) Axial length of the eyeball changes with aging in the mice strains of (e) C57BL/6 and (f) Balb/c, respectively. Shaded area represents the standard deviation. Number of mice ≥3, except 60 and 62 weeks (n = 2).