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. 2021 Apr 16;62(4):21. doi: 10.1167/iovs.62.4.21

Figure 2.

Figure 2.

EMT of RPE is activated in culture time-dependent manner. (a) Representative images of RPE cells cultured at 50% and 12.5% confluence for one, three, six, nine, and 14 days. Scale bar: 100 µm. (b–c) The mRNA expression levels of the RPE markers OTX2, MITF, RPE65, Best, CRALBP, and PMEL and the EMT markers FN1, PAI-1, α-SMA, Col IV, Zeb-1, and N-Cadherin were tested by RT-PCR in RPE cells cultured at 50% (b) and 12.5% (c) confluence for one, three, six, and nine days (n = 3 per group with duplicates). *P < 0.05, **P < 0.01 versus the one-day group. Data were normalized to GAPDH. (d) Western blotting bands showing the expression of RPE65, CRALBP, E-Cadherin, FN1, and α-SMA in RPE cells cultured at 12.5% confluence for one, three, six, nine, and 14 days. RPE cells cultured at 50% confluence for 14 days were used as the mature RPE control. The results are representative of at least three independent experiments.