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. 2021 Apr 15;11:632540. doi: 10.3389/fonc.2021.632540

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Copyright © 2021 Guo, Sun, Qian, Zhu, Ta, Wang, Zheng, Guo and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation and characterization of feeder cells expressing secretory Neoleukin-2/15 (*p<0.05, ***p<0.001; NS indicates no significant difference). (A) In vitro expansion of the NK cells from 5 volunteers by IL-2 or Neo-2/15 at the indicated concentration. (B) Genetically Engineered K562 or NK92 clones expressing secretory neoleukin-2/15 were analyzed by flow cytometry. (C) The survival of irradiated clones cultured in AIM-V medium and examined over one week. (D) Neo-2/15 secretion by indicated clones was measured. (E) NK cells were co-cultured with irradiated K562-Neo2/15 or NK92-Neo2/15, cell expansion fold was measured. (F) Cytotoxicity assay to measure NK-mediated cell killing in Raji cell at indicated effector-to-target ratios.