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. 2021 Apr 15;11:632540. doi: 10.3389/fonc.2021.632540

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Copyright © 2021 Guo, Sun, Qian, Zhu, Ta, Wang, Zheng, Guo and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NK92-Neo2/15-OX40L promotes an OX40-OX40L positive feedback loop (*p < 0.05). (A) Western-blot detection of NK-κB activation for indicated treatment. (B) 4-1BB and OX40 expression in expanded NK cells from 5 donors, measured by Western-blot before and after feeder cell treatment. (C) Relative gray value statistics of protein level in (B). (D) Immunoblot of TRAF5, phosphorylated-p65, and p65 was detected. (E) FACS analysis of OX40L expression on expanded NK. (F) NK cells from PBMC, treated by IL-2 or NK92-Neo2/15-OX40L expanded NK cells at a ratio of 19:1, freshly collected NK cells were used as control. Phosphorylated p65 were determined by western blot.