Figure 4.

Relationship between total and subgenomic RNA in a persistently infectious patient. Total N was expressed at a 11-fold (3.5 standard deviation {SD}, 0.75] cycles) higher level than subgenomic N (sgN), and total E was expressed at an 84-fold (6.4 [SD, 0.8] cycles) higher level than subgenomic E (sgE) when all time points were combined (A). Time course showing 45 minus cycle thresholds (Ct) for total (black) and subgenomic RNA (red) over time. This patient showed persistently positive total and subgenomic RNA for 119 days. The ratio of total N and sgN of 3 to 4 cycles was maintained over the duration of infection (B). This was also observed for E and sgE with the ratio of 6 to 7 cycles maintained thorough the course of infection (C).