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. 2021 Jan 9;24(3):586. doi: 10.4103/jomfp.JOMFP_49_20

Table 2.

Staining adequacy of the tissue sections from the old tissue blocks, long term formalin fixed tissues and freshly fixed tissues in H&E, Mallory’s and Van Gieson’s stain

Staining adequacy OTB LFT FFT



H&E, n (%) Mallory, n (%) VG, n (%) H&E, n (%) Mallory, n (%) VG, n (%) H&E, n (%) Mallory, n (%) VG, n (%)
Very good 13 (25.50) 5 (9.80) 2 (3.90) 1 (2.00) 0 (0.00) 0 (0.00) 32 (62.70) 32 (62.70) 21 (41.20)
Good 35 (68.60) 36 (70.60) 31 (60.80) 12 (23.50) 15 (29.40) 7 (13.7) 19 (37.30) 18 (35.30) 28 (54.90)
Satisfactory 2 (3.90) 8 (15.70) 14 (27.50) 29 (56.90) 34 (66.70) 30 (58.8) 0 (0.00) 0 (0.00) 2 (3.90)
Inadequate 1 (2.00) 2 (3.90) 4 (7.80) 9 (17.60) 2 (3.90) 14 (27.5) 0 (0.00) 1 (2.00) 0 (0.00)
Total 51 (100) 51 (100) 51 (100) 51 (100) 51 (100) 51 (100) 51 (100) 51 (100) 51 (100)
Chi-square test between three groups 21.11 14.05 11.65
P 0.002 S 0.003 S 0.07 NS

S: Significant, NS Not significant, VG: Van Gieson’s, OTB: Old tissue blocks, LFT: Long term fixed tissues, FFT: Freshly fixed tissues