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miR-486 modulates the expression of cyclins and CDK4. (A) Relative expression of CDK4, CCND1 and CCNE1 following transfection with miR-486 mimic. Expression values refer to the control condition (miRNA control=1). Statistical significance is indicated vs. the control: *P<0.05. (B) Western blot analysis of CDK4, CCND1 and CCNE1 in cellular extracts following 72 h of transfection.
