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. 2021 Apr 28;47(6):117. doi: 10.3892/ijmm.2021.4950

Figure 4.

Figure 4

Transfection with miR-486 mimic modulates the expression of PIM1 and the release of inflammation factors. (A) Relative expression of PIM1 following transfection. Expression values refer to the control condition (miRNA control=1). (B) Western blot analysis of PIM1 protein following 24 h of transfection. (C) Concentration of IL-6 in culture medium of transfected cells at 24 h. Comparable results were also obtained 48 and 72 h post-transfection (data not shown). Values refer to the control condition (miRNA control=100%). Statistical significance is indicated vs. the control: *P<0.05; **P<0.01; ***P<0.001. PIM1, provirus integration site for Moloney murine leukaemia virus 1.