Figure 6.

Transfection with miR-486 mimic increases the apoptosis of CDDP-treated cells. Exponentially growing H2052 and H28 cells were transfected and/or treated for 48 h with CDDP at the respective IC₅₀ value. (A) Flow cytometry dot plots represent the results of cells staining with Annexin V-FITC/PI. (B) Apoptotic cell percentages. (C) Caspase-3 activity was measured at 24 h following CDDP treatment. Values refer to the control condition (miRNA control). Statistical significance is indicated as follows: CDDP vs. CDDP + miR-486: ^*P<0.05; ^**P<0.01. CDDP, cisplatin.