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. 2021 Apr 15;12:662352. doi: 10.3389/fmicb.2021.662352

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Copyright © 2021 Peng, Bu, Yin, Hua, Zhao, Lu, Zheng, Wan, Zhang, Chen, Liu, Chen, Mo and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PoPeVYVaRNA is responsible for the synergism with PoPeVYV in N. benthamiana. (A) The PoPeVYVaRNA mutant infectious clones prepared. (B) Relative fold changes of PoPeVYV in local leaves of N. benthamiana co-inoculated with PoPeVYVaRNA or mutants of PoPeVYVaRNA (pCB301-MD as control), as shown by quantitative real-time reverse transcription PCR. The means (±SE) were calculated from the RNA levels of six individual plants at 3 days post inoculation. (C) Phenotype of N. benthamiana plants agroinfiltrated with viral infectious clone combinations or empty agrobacterium (CK) at 14 days post infiltration. RT-PCR confirming the presence of viral RNAs in local/systemic leaves of inoculated plants. 1, negative control; 2, positive control; 3, CK; 4, PoPeVYV + pCB301-MD; 5, PoPeVYV + PoPeVYVaRNA-orf1a; 6, PoPeVYV + PoPeVYVaRNA-orf1b; 7, PoPeVYV + PoPeVYVaRNA-orf1ab; 8, PoPeVYV + PoPeVYVaRNA.