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. 2021 Apr 15;9:642378. doi: 10.3389/fcell.2021.642378

FIGURE 6.

FIGURE 6

Modeling SNX27/retromer on membranes. Thermophilic yeast SNX-BAR/retromer (PDB ID: 6H7W) (A) and mammalian SNX3/retromer coats (B) (PDB: not yet available) have been reconstituted in vitro. The view in panel (B) was generated using PDB: 5F0J, which approximates the reported SNX3/retromer architecture. Both complexes drive tubulation, and reconstructions indicate retromer forms conserved asymmetrical V-shaped arches across eukaryotes. VPS35 is shown as red ribbons, VPS26 as blue ribbons, VPS29 as green ribbons, Vps5 as yellow ribbons, and the SNX3 PX domain as gray ribbons. On each model, potential locations for SNX27 domains are marked. In the SNX-BAR/retromer model (A), the SNX27 PX domain (gray ribbons) appears to be occluded by BAR dimers, and the PDZ domain would likely be blocked from engaging membrane cargo by the BAR layer. In the SNX3/retromer model (B), the SNX27 PX (gray ribbons) and PDZ (purple ribbons) domains are both located close to the membrane. There are currently no structural data regarding the overall architecture of SNX27, either on its own or as part of a retromer coat, so the location of the FERM domain remains unknown. Both models assume the SNX27 PX occupies a similar location to SNX3 PX.