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. 2021 Apr 15;9:656359. doi: 10.3389/fcell.2021.656359

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Copyright © 2021 Shen, Huang, Wu, Li, Hu, Wang, Zhang, Shao and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SCRIB promotes the migration and invasion of CRC cells in vitro. SCRIB was knocked down and overexpressed in HCT116 and DLD-1 cells, respectively. The efficiency of knockdown and overexpression was verified by qRT-PCR and WB (A,B). (C) Effects of SCRIB on migration in CRC cell lines were detected by wound healing assay, scale bars: 100 μm. (D) Cell migration and invasion of HCT116 and DLD-1 cells as measured by Transwell assays; scale bars: 100 μm. (E) Western blot analysis of the tight junction protein ZO-1, epithelial marker E-cadherin, and mesenchymal markers N-cadherin and Vimentin after SCRIB knockdown in HCT116 cells. (F) The same molecules were observed in DLD-1 cells overexpressing SCRIB. The data represent the mean ± SD from three independent experiments. Student’s t-test was used to determine statistical significance: *P < 0.05, **P < 0.01, and ***P < 0.001.