FIGURE 4.

SCRIB enhances tumour growth in vivo. (A) Nude mice were subcutaneously injected with HCT116 cells transfected with lentivirus and DLD-1 cells transfected with overexpression plasmid and their corresponding control cells. The established tumours in the right and left groins of the mice were measured every 5 days, and the mice were sacrificed 4–6 weeks after implantation. (B) Knockdown of SCRIB inhibited the tumour growth of HCT116 cells in nude mice, while overexpression of SCRIB promoted the tumour growth of DLD-1 cells in nude mice. (C) Apoptotic cells in tumour tissue were detected by TUNEL assay, and the number of TUNEL-positive cells was quantified; scale bars: 50 μm. (D) Protein levels of Ki67 in the tumour samples were determined by IHC, scale bars: The data are shown as the mean ± SD of three independent experiments, *P < 0.05, **P < 0.01.