Table 4. Variation of the HA 222D/G/N polymorphism in the A(H1N1)pdm09 viruses in primary material (pm) and isolated on MDCK.
| strain | Tissue | GAT (D) original % | GAT (D) MDCK % | Ratio (original to MDCK) | GGT (G) original % | GGT (G) MDCK % | Ratio (MDCK to original) | AAT (N) original % | AAT (N) MDCK % | Ratio (MDCK to original) | Original sample, coverage# | MDCK C1, passage coverage# |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A/Russia/261/2019 | l | 0.2 | 0.2 | 1.0 | 98.5 | 97.8 | 1.0 | 0 | 0 | No | 100130 | 84560 |
| A/Vologda/332/2019 | l | 76.1 | 0.2 | 380.5 | 23.1 | 97.4 | 4.2 | 0.1* | 0 | No | 50419 | 94217 |
| A/Yekaterinburg/322/2019 | tr,br,l | 86.9 | 2.8 | 31.0 | 11.1 | 84.8 | 7.6 | 1.1 | 10.1 | 9.2 | 66921 | 108254 |
| A/Yekaterinburg/72/2019 | l | 57.8 | 0.3 | 192.7 | 22.6 | 81.6 | 3.6 | 18 | 15.8 | 0.9 | 74916 | 115634 |
| A/Irkutsk/973/2019 | l | 65.5 | 8.7 | 7.5 | 12.5 | 67.6 | 5.4 | 21.1 | 21.7 | 1.0 | 27665 | 98692 |
| A/Samara/483/2019 | l | 65.6 | 17.5 | 3.7 | 27.4 | 29.9 | 1.1 | 6 | 50.5 | 8.4 | 57505 | 92736 |
| A/Khabarovsk/31/2019 | l | 98.1 | 68.6 | 1.4 | 0.8 | 29.6 | 37.0 | 0.2 | 0.2 | 1.0 | 13840 | 109720 |
| A/Moscow/1/2019 | swab | 75.4 | 24.2 | 3.1 | 11.5 | 24.1 | 2.1 | 11.6 | 49.2 | 4.2 | 99268 | 66943 |
| A/Irkutsk/1061/2019 | l | 98.5 | 84.6 | 1.2 | 0.6 | 8.7 | 14.5 | 0.1* | 5.2 | 52.0 | 28140 | 111914 |
| A/Irkutsk/864/2019 | l | 98.7 | 94.3 | 1.0 | 0.4 | 4.3 | 10.8 | 0.2 | 0.1* | No | 48521 | 79049 |
| A/Astrakhan/2/2019 | l | 77.3 | 48.1 | 1.6 | 0.3 | 3.7 | 12.3 | 9.1 | 7.9 | 0.9 | 58798 | 127410 |
| A/Astrakhan/3/2019 | tr,l | 98.7 | 96.2 | 1.0 | 0.4 | 0.8 | 2.0 | 0.1 | 1.3 | 13.0 | 38477 | 146680 |
The table shows the percentage of virus variants with the mutations in the sample.
*-value is below detection limit.
#-coverage is the total number of NGS sequences (reads) at amino acid position 222 in HA (l–lung, tr–trachea, br–bronchi).