Fig. 2.

(A) Sequences of peptide MBP81-104 and its variants with point amino acid substitutions to alanine. Point substitutions are indicated with different colors. (B, C) Binding of peptide MBP81-104 and its variants (750 nM) carrying point amino acid substitutions to alanine with CLIP-bound HLA-DR1 (B) and HLA-DR15 (C) (150 nM). Column colors correspond to the colors of point substitutions. White bars represent the background signal (PBS). Thioredoxin (TRX) with a linker was used as a negative control. Standard deviation is presented