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. Author manuscript; available in PMC: 2021 Apr 29.
Published in final edited form as: J Comp Neurol. 2016 Nov 21;525(5):1291–1311. doi: 10.1002/cne.24137

Figure 4. In CB2SH3− overexpressing neurons, gephyrin superclusters are associated with presynaptic vGAT puncta. However there is no effect on the extent or fluorescence intensity of the vGAT puncta contacting the transfected neuron.

Figure 4.

(A-F) Triple-label fluorescence with anti-Geph (blue), anti-vGAT (red) and EGFP (green) at P45. Arrows and arrowheads show some examples of apposition between Geph superclusters and vGAT in somas (arrows) and dendrites (arrowheads). B and E show red and blue overlays while C and F show RGB overlays. (G-J) Double-label fluorescence with anti-vGAT (red) and EGFP (green). The extent and fluorescence intensity of vGAT puncta contacting CB2SH3− transfected neurons (green, asterisks H and J) and non-transfected neurons (numbers 1 and 2) are similar; Scale bar = 10 μm in A-F and 19 μm in G-J.