Figure 5. Quantification of the effect of CB2_SH3− overexpression on vGAT clusters.

The box plots display the median and interquartile range of the data while the whiskers represent the spread of the data within the 1.5 interquartile ranges of the upper and lower quartile. Inside the boxes, circles indicate mean and bars from mean indicate 95% confidence interval. (A) Mean fluorescence intensity per pixel of vGAT immunofluorescence in CB2_SH3− overexpressing neurons normalized to the corresponding mean value from sister non-transfected neurons at P44-P45. P=0.614 (Mann-Whitney U=263, n₁=n₂=24 neurons). (B) Size of vGAT clusters in the somas of CB2_SH3− overexpressing neurons compared to sister non-transfected controls P=0.515 (Mann-Whitney U=108,869, n₁=496 clusters, n₂=450 clusters). (C) Number of vGAT clusters in the CB2_SH3− overexpressing neurons compared to sister non-transfected controls P=0.597 (Mann-Whitney U=136.5 n₁=18 neurons, n₂=17 neurons).