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. Author manuscript; available in PMC: 2021 Apr 29.
Published in final edited form as: J Comp Neurol. 2016 Nov 21;525(5):1291–1311. doi: 10.1002/cne.24137

Figure 9. In CB2SH3+ overexpressing neurons, gephyrin clusters are associated with presynaptic vGAT puncta. There is no effect on the extent or fluorescence intensity of the vGAT puncta contacting the transfected neuron.

Figure 9.

(A-C and D-F) Triple-label fluorescence with anti-Geph (blue), anti-vGAT (red) and EGFP (green) at P42 cortical neurons. Arrows and arrowheads show some examples of apposition of Geph clusters and vGAT in somas (arrows) and dendrites (arrowheads). C and F show the RGB overlays. For comparative purposes A-C shows a transfected neuron (green, asterisk) and a non-transfected sister neuron (number 1). (G and H) Double-label fluorescence. The extent and fluorescence intensity of vGAT puncta (red) contacting CB2SH3+ transfected neurons (green, asterisks) and non-transfected neurons (numbers 1–3) are similar. Note that the transfected neurons (green) in G and H are the same as in Fig 7A and B. For comparative purposes $ symbols were added corresponding to the cells labelled with the $ symbol in Fig 7A and B. Scale bar = 10 μm in A-F and 17 μm in G and H.