Table 4. Analysis of ICeChIP Calibrant Barcodes.
| On-target IP/Input Ratio: | Specificity Plot: | |||||||
|---|---|---|---|---|---|---|---|---|
| Sample | Series | Barcodes | Uniread | SmartMap | Species | Slope | R2 | |
| AR7 | H3K4me3 Rep. 1 | Ser. 1 | 11 | 19.88 | 20.05 | 1 | – | – |
| H3K4me3 Rep. 2 | Ser. 1 | 11 | 18.95 | 18.99 | 1 | – | – | |
| AR8 | H3K27me3 | Ser. 2 | 100 | 0.877 | 0.879 | 1 | – | – |
| AR9 | H3K4me3 | Ser. 2 | 100 | 27.7 | 28.3 | 7 | 1.051 | 0.9984 |
| H3K9me3 | Ser. 2 | 100 | 1.34 | 1.26 | 7 | 1.012 | 0.9972 | |
| H3K27me3 | Ser. 2 | 100 | 0.678 | 0.677 | 7 | 1.022 | 0.9995 | |
| AR16 | H3K4me1 | Ser. 3 | 136 | 4.34 | 4.84 | 17 | 1.005 | 0.9967 |
| H3K4me2 | Ser. 3 | 136 | 3.98 | 3.75 | 17 | 1.004 | 0.9992 | |
| H3K4me3 | Ser. 3 | 136 | 32.4 | 31.1 | 17 | 1.009 | 0.9987 | |
| AR17 | H3K9me3 | Ser. 3 | 136 | 2.45 | 2.23 | 17 | 1.005 | 0.9996 |
| H3K27me3 | Ser. 3 | 136 | 1.82 | 1.73 | 17 | 1.002 | 0.9998 | |
Barcodes: the number of unique DNA barcode sequences in the ICeChIP calibrant series.
Species: the number of distinct modified nucleosomes marked by the barcodes, including the target modification and, if there is more than one species, the off-target modifications.