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. 2021 Mar 28;14(3):dmm048922. doi: 10.1242/dmm.048922

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Expression of serotonin in the small intestine of NAFLD/NASH mice models. (A) Representative images of serotonin expression in the small intestine of the model mice. Five different sections from each of the five mice (n=25) in all groups were quantitatively analyzed for the positively stained area using ImageJ software. (B) Expression of tryptophan hydroxylase 1 in the small intestine. Gapdh was used as an internal control. Data are mean±s.d. *P<0.05; **P<0.01; N.S., not significant (paired two-tailed Student's t-test).