Figure 3.

miR-99a was negatively regulated by THRIL. A. The predicted banding sequences of THRIL and miR-99a; B. H9C2 cells were co-transfected with the luciferase reporter plasmid containing wild-type or mutant THRIL and miR-99a mimic or mimic control. Dual luciferase activity assay was performed to detect the interaction between miR-99a and THRIL; C. The expressions of miR-99a in H9C2 cells were assessed by quantitative real time polymerase chain reaction after transfection with sh-THRIL or sh-NC; *p < 0.05; **p < 0.01.