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. 2021 May;27(5):1427–1437. doi: 10.3201/eid2705.190121

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ELISA for ZIKV NS1 detection in study of Zika diagnosis, Singapore. A) Receiver operating characteristics curve analysis showing the performance of C12-C11 sandwich ELISA when tested against ZIKV-infected or non–ZIKV-infected samples. B) ZIKV NS1 quantification in patient samples using in-house antibody pairs. Each dot represents an individual patient sample. C) Distribution of number of plasma cases (x-axis) over dpo (y-axis) for ZIKV NS1 ELISA tested with the validation set; positive plasma (black) and the total plasma cases (gray) at each dpo are also shown. DENV, dengue virus; dpo, days postonset of symptoms; NS1, nonstructural protein 1; ZIKV, Zika virus.