Table 1. Overview of SARS-CoV-2 wastewater samples sequenced during study of circulation and diversity through community wastewater sequencing, the Netherlands and Belgium*.
| Sample no. |
Sample ID |
Date |
Country |
Sampling location |
Target Ct† |
|
Coverage, % |
||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| N1 |
N2 |
N3 |
E |
Nanopore |
Illumina |
||||||
| 1 | 92499 | 2020 Mar 25 | Netherlands | Heeswijk-Dinther | 32.9 | 32.1 | 30.7 | 30.8 | 94.4 | ND | |
| 2 | 92502 | 2020 Mar 25 | Netherlands | Apeldoorn | 36.6 | 34.9 | 33.2 | 33.3 | 74.9 | 19.4 | |
| 3 | 92503 | 2020 Mar 25 | Netherlands | Amersfoort | 34.9 | 33.1 | 31.8 | 32.1 | 87.8 | ND | |
| 4 | 92504 | 2020 Mar 25 | Netherlands | Utrecht | 31.8 | 30.9 | 29.8 | 29.9 | 95.2 | ND | |
| 5 | 92505 | 2020 Mar 25 | Netherlands | Utrecht Overvecht | 32.3 | 31.1 | 30.1 | 30.1 | 92.4 | ND | |
| 6 | 92506 | 2020 Mar 25 | Netherlands | Schiphol | 32.7 | 32.0 | 30.8 | 30.7 | 92.2 | 65.6 | |
| 7 | 92508 | 2020 Mar 25 | Netherlands | Amsterdam West | 31.8 | 30.7 | 29.7 | 29.9 | 97.0 | ND | |
| 8 | 92509 | 2020 Mar 25 | Netherlands | Tilburg | 33.0 | 32.2 | 31.2 | 31.0 | 78.8 | 65.5 | |
| 9 | 92719 | 2020 Mar 30 | Netherlands | Franeker | 31.8 | 30.8 | 31.2 | 30.7 | 97.7/50.9‡ | 78.2 | |
| 10 | 92721 | 2020 Mar 30 | Netherlands | Beverwijk | 32.6 | 31.4 | 31.8 | 30.8 | 93.7 | 47.7 | |
| 11 | 92722 | 2020 Mar 30 | Netherlands | Katwoude | 32.9 | 32.6 | 32.7 | 31.4 | 84.6 | 53.9 | |
| 12 | 92723 | 2020 Mar 30 | Netherlands | Wervershoof | 33.1 | 32.3 | 32.5 | 31.1 | 96.6 | 43.2 | |
| 13 | 92848 | 2020 Apr 1 | Netherlands | Amersfoort | 33.6 | 32.1 | 32.3 | 31.6 | 96.6 | 39.4 | |
| 14 | 92849 | 2020 Apr 1 | Netherlands | Utrecht | 32.4 | 31.4 | 31.8 | 30.6 | 57.8 | 48.5 | |
| 15 | 92851 | 2020 Apr 1 | Netherlands | Schiphol | 33.7 | 33.1 | 33.4 | 32.3 | 89.4 | 53.5 | |
| 16 | 92852 | 2020 Apr 1 | Netherlands | Amsterdam West | 31.8 | 30.6 | 30.9 | 29.9 | 99.2/97.1‡ | 59.1 | |
| 17 | 92853 | 2020 Apr 1 | Netherlands | Tilburg | 33.5 | 32.6 | 32.6 | 32.0 | 91.2 | ND | |
| 18 | 92943 | 2020 Apr 2 | Belgium | Langemark | 33.2 | 33.3 | 33.1 | 32.2 | 60.3 | ND | |
| 19 | 92947 | 2020 Apr 2 | Belgium | Lo-Reninge | 34.6 | 34.2 | 34.5 | 33.4 | 71.3 | ND | |
| 20 | 92949 | 2020 Apr 2 | Belgium | Properinge | 34.5 | 33.4 | 33.4 | 32.4 | 65.6 | 65.6 | |
| 21 | 92965 | 2020 Apr 2 | Netherlands | Delft | 32.9 | 32.9 | 32.9 | 31.5 | 91.7 | 52.4 | |
| 22 | 93030 | 2020 Apr 5 | Belgium | Aartselaar | 33.2 | 32.4 | 31.6 | 31.4 | 89.9 | 61.2 | |
| 23 | 93032 | 2020 Apr 5 | Belgium | Gent | 34.2 | 33.7 | 32.6 | 32.1 | 63.2 | 46.9 | |
| 24 | 93034 | 2020 Apr 5 | Belgium | Leuven | 33.6 | 33.4 | 32.1 | 31.4 | 70.2 | 37.6 | |
| 25 | 93036 | 2020 Apr 5 | Belgium | Tienen | 33.3 | 32.6 | 31.2 | 30.8 | 88.1 | 41.5 | |
| 26 | 93818 | 2020 Apr 8 | Netherlands | Amersfoort | 34.9 | 34.3 | 33.4 | 32.4 | 37.5 | ND | |
| 27 | 93820 | 2020 Apr 9 | Netherlands | Utrecht | 32.8 | 32.2 | 31.2 | 30.8 | 55.2 | ND | |
| 28 | 93822 | 2020 Apr 9 | Netherlands | Amsterdam West | 32.6 | 25.1 | 31.6 | 30.9 | 87.3 | ND | |
| 29 | 93823 | 2020 Apr 9 | Netherlands | Schiphol | 33.0 | 33.2 | 32.2 | 31.3 | 67.3 | 43.5 | |
| 30 | 93825 | 2020 Apr 8 | Netherlands | Delft | 33.9 | 33.7 | 32.7 | 32.0 | 63.9 | 64.3 | |
| 31 | 93828 | 2020 Apr 9 | Netherlands | Tilburg | 35.2 | 34.6 | 33.1 | 32.7 | 31.2 | ND | |
| 32 | 93948 | 2020 Apr 14 | Netherlands | Heeswijk-Dinther | 35.8 | 34.6 | 33.6 | 32.7 | 18.8 | ND | |
| 33 | 93950 | 2020 Apr 15 | Netherlands | Wervershoof | 34.9 | 34.3 | 33.1 | 32.5 | 60.7 | ND | |
| 34 | 94330 | 2020 Apr 21 | Netherlands | Utrecht1 | 35.1 | 34.4 | 33.2 | 33.5 | 41.7 | ND | |
| 35 | 94331 | 2020 Apr 21 | Netherlands | Utrecht2 | 35.7 | 34.1 | 34.2 | 33.7 | 38.6 | ND | |
| 36 | 94334 | 2020 Apr 21 | Netherlands | Amsterdam West | 34.0 | 33.3 | 32.4 | 32.0 | 66.7 | ND | |
| 37 | 94335 | 2020 Apr 21 | Netherlands | Schiphol | 33.8 | 34.1 | 32.9 | 33.7 | 40.1 | 43.0 | |
| 38 | 94337 | 2020 Apr 21 | Netherlands | Delft | 35.7 | 34.1 | 34.1 | 33.8 | 34.2 | ND | |
| 39 | 94339 | 2020 Apr 21 | Netherlands | Tilburg | 34.8 | 35.4 | 34.7 | 36.0 | 11.2 | 80.3 | |
| 40 | 94602 | 2020 Apr 29 | Netherlands | Utrecht | 35.6 | 34.3 | 33.0 | 34.2 | 29.6 | ND | |
| 41 | 94604 | 2020 Apr 29 | Netherlands | Amsterdam West | 34.9 | 34.6 | 32.8 | 33.6 | 15.0 | ND | |
| 42 | 94605 | 2020 Apr 29 | Netherlands | Schiphol | 34.6 | 35.1 | 33.6 | 33.2 | 21.3 | 35.2 | |
| 43 | 94607 | 2020 Apr 25 | Netherlands | Delft | 35.8 | 36.2 | 34.4 | 34.0 | 15.3 | ND | |
| 44 | 94976 | 2020 May 7 | Netherlands | Utrecht | 35.5 | 36.0 | 35.1 | 33.5 | 6.3 | ND | |
| 45 | 94978 | 2020 May 7 | Netherlands | Amsterdam West | 35.0 | 34.8 | 34.5 | 33.7 | 19.8 | ND | |
| 46 | 94982 | 2020 May 6 | Netherlands | Delft | 35.1 | 35.9 | 34.7 | 33.7 | 18.7 | ND | |
| 47 | 95550 | 2020 May 13 | Netherlands | Utrecht | ND | 34.4 | ND | 32.0 | 3.0 | ND | |
| 48 | 95552 | 2020 May 13 | Netherlands | Amsterdam West | ND | 34.2 | ND | 32.8 | 20.4 | ND | |
| 49 | 95556 | 2020 May 12 | Netherlands | Delft | ND | 34.4 | ND | 34.1 | 0 | ND | |
| 50 | 95558 | 2020 May 13 | Netherlands | Tilburg | ND | 34.3 | ND | 36.1 | 0 | ND | |
| 51 | 95793 | 2020 May 19 | Netherlands | Utrecht | ND | 35.1 | ND | 34.9 | 0 | ND | |
| 52 | 95794 | 2020 May 19 | Netherlands | Amsterdam West | ND | 35.1 | ND | 34.2 | 7.7 | ND | |
| 53 | 96925 | 2020 Jun 2 | Netherlands | Utrecht | ND | 35.2 | ND | 37.1 | 0 | ND | |
| 54 | 96927 | 2020 Jun 2 | Netherlands | Schiphol | ND | 32.5 | ND | 31.1 | 30.8 | 34.0 | |
| 55 | 97044 | 2020 Jun 3 | Netherlands | Delft | ND | 35.7 | ND | 33.5 | 8.2 | ND | |
*Ct, cycle threshold; E, envelope; N, nucleocapsid; ND, not determined; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. †Three primer–probe sets targeting the N1–N3 genes and 1 targeting the E gene. ‡These samples were sequenced twice.