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. 2021 May;27(5):1362–1370. doi: 10.3201/eid2705.204055

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ELISA reactivities against different antigens of pre–coronavirus disease (COVID-19) cat and dog serum samples and paired samples of FCoV type I infection, the Netherlands. A) Reactivities of pre–COVID-19 cat serum samples against SARS-CoV-2 S1, RBD, N, and FCoV type I S1. B) Reactivities of pre–COVID-19 dog serum samples against SARS-CoV-2 S1, RBD, N, BCoV S1, and FCoV type II S1. C) Reactivities of paired SPF cat serum samples (left panel) and FCoV type I–specific serum samples (right panel) to SARS-CoV-2 S1, subunit; RBD, N, and FCoV S1 protein levels were determined by ELISA. Dotted lines indicate positive cutoff levels. BCoV, bovine coronavirus; FCoV, feline coronavirus; N, nucleocapsid; OD, optical density; RBD, receptor-binding domain; S1, spike protein subunit 1; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; SPF, specific pathogen free.