Geographic coverage and serologic analysis of cat (A, C) and dog (B, D) samples of 2020 cohorts for SARS-CoV-2, the Netherlands. A, B) Geographic distribution. Choropleth maps were produced by using ArcGIS version 9.3.1 (Esri, https://www.esri.com). C, D) ELISA and VNT analysis. Number and percentages of positive samples are indicated. Dotted lines indicate positive cutoff levels. Samples that had a VNT >16 were considered positive. IC50, 50% inhibitory concentration; OD, optical density; RBD, receptor-binding domain; S1, spike protein subunit 1; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; VNT, virus neutralization titer.