Fig. 2.

TAZ, but not YAP, is required for thrombin-mediated activation of the Hippo pathway in invasive breast cancer cell lines. (A) Equivalent amounts (20 µg) of cell lysates from various breast cancer cell lines were immunoblotted (IB) for Hippo pathway components: YAP, TAZ, LATS1, MST1, MST2, β-TRCP (also known as BTRC), SAV1 and MOB1 expression. β-actin expression was used as a control. (B) YAP (red) and TAZ (blue) mRNA expression in invasive breast cancer cell lines was quantified by qPCR. Results are normalized to S18 mRNA expression and are represented as the fold-change relative to YAP expression in parental MDA-MB-231. Data are mean±s.d., n=3. Statistical significance was determined by two-way ANOVA with Tukey′s post hoc test. MDA-MB-231 HA–ARRDC3 pSLIK (C–E) and Hs578T (F,G) cells were transfected with the indicated siRNAs and stimulated with 10 nM α-thrombin (α-Th). Results are represented as the fold increase in CTGF expression (D,G) and ANKRD1 expression (E) relative to 0 min non-specific siRNA (NS)-transfected control. Data are mean±s.d., n=3. Statistical significance was determined by two-way ANOVA with Tukey′s post hoc test. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.