Fig. 4.

ARRDC3 re-expression suppresses GPCR activation of the Hippo pathway. (A–D) ARRDC3 expression was induced by doxycycline (DOX) treatment in MDA-MB-231 HA–ARRDC3 pSLIK cells, then stimulated with 10 nM α-thrombin (α-Th; A), 100 nM LPA (B), 1 µM SLIGKV (C) or 100 nM S1P (D). Samples were immunoblotted (IB) with antibodies against the indicated proteins. Results are the fold increase in CTGF and ANKRD1 expression relative to 0 min −DOX control. Data are mean±s.d., n=3. Statistical significance was determined by two-way ANOVA with Tukey′s post hoc test. *P<0.05; **P<0.01; ***P<0.001.