Figure 3.

Xi Lei San prevented the activation of NLRP3 inflammasomes and autophagy in rats with DSS-induced colitis. (a) The DSS model rats with ulcerative colitis were treated with low or high doses of Xi Lei San. NLRP3, IL-1β, caspase-1, and ASC expression determined by western blotting. GAPDH served an internal reference protein. (b) ELISA kits were used to analyze the concentrations of IL-1β, IL-18, and IL-33 in each group. (c) Autophagy-related proteins, LC3B and p62, were measured by western blotting. ^∗P < 0.05 and ^∗∗P < 0.01 vs. the control group; ^#P < 0.05 and ^##P < 0.01 vs. the model group.