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. 2021 Apr 6;40(17):3164–3179. doi: 10.1038/s41388-021-01762-0

Fig. 6. LINC01133 promotes EMT through the Wnt/β-catenin signaling pathway.

Fig. 6

A The FISH results suggested that LINC01133 transcripts were more abundant in the nucleus than in the cytoplasm. B The LINC01133-associated gene is mainly enriched in the Wnt pathway by KEGG analysis. C The expression change of E-cadherin and Vimentin proteins after adding LiCl in Si-LINC01133 transfected SW1990 cells. D Periostin can promote EGFR-Erk-c-myc signaling. E ChIP-PCR showed that c-myc occupied the LINC01133 promoter region. F The binding of c-myc at LINC01133 promoter region was enhanced by PN stimulation by ChIP-qPCR. The experiment was repeated three times, and the significance was analyzed by a Student’s t test. Data are shown as mean ± SD (**P < 0.01 vs. control). G ChIRP was used to examine the association of LINC01133 and biotin probes in different LINC01133 treatment groups. H ChIRP was used to examine the association of LINC01133 and EZH2 in different LINC01133 treatment groups. I Protein levels of EZH2 were positively associated with LINC01133 expression. J ChIP-qPCR suggests that LINC01133 promotes H3K27Me3 occupation of the AXIN2 promoter regions. The experiment was repeated three times, and the significance was analyzed by Student’s t test. Data are shown as mean ± SD (**P < 0.01, *P < 0.05 vs. NC). K LINC01133 was negatively related with AXIN2 and positively related with β-catenin and c-myc. L Downregulated LINC01133 triggered a reduction of nuclear and cytoplasmic β-catenin and p-β-catenin (Ser675) protein levels. H3 was used as the nuclear control, and β-actin was used as the cytoplasmic control. M The time course elucidated Si-LINC01133 and Lv-LINC01133 induces or inhibits AXIN2 expression at about 36 h and β-catenin can be upregulated or downregulated by Lv-LINC01133 and Si-LINC01133 also at about 36 h.