Fig. 5.

UCP2 deficiency enhanced NLRP3 inflammasome activation in HT22 cells under OGD/R and high glucose conditions. a Double immunostaining of NLRP3, ASC, Caspase 1, Cleaved Caspase 1, and IL-1β (red) with DAPI (blue)) was performed in the different groups. b Representative western blots showing NLRP3, ASC, Caspase 1, Cleaved Caspase 1, TXNIP, and IL-1β in the different groups. c–h Quantification of the average NLRP3, ASC, Caspase 1, Cleaved Caspase 1, TXNIP, and IL-1β expression normalized to β-actin. i The level of IL-1β in HT22 cell culture medium. The experiments were repeated three times in triplicate for each condition. ^#p < 0.05 vs. Control; *p < 0.05 vs. HG; ^$p < 0.05 vs. NG + OGD/R; ^@p < 0.05 vs. HG + OGD/R; ^%p < 0.05 vs. NG + OGD/R + GE