Fig. 1.

Key-enzymes of glycogen metabolism and glycogen content in cultured mouse neural cell types. a,b Western blot analysis for the detection of glycogen phosphorylase brain isoform (GPBB) and glycogen synthase (GS) in homogenates of NSC-34 and N18TG2 cells compared to neuronal primary cultures (NPC) and pure motoneuron cultures. c Western blot analysis of differentiated NSC-34 cells compared to undifferentiated cultures. Amount of protein applied per lane: a 10 µg, b 5 µg, c 30 µg for GPBB and 15 µg for GS. d Quantitative determination of glycogen. Data represent mean values ± SD of n experiments each performed in triplicate. Values for NPC were taken from the literature. Here, error bar could not be indicated because individual values were not reported [9]