Fig. 3.

Influence of glucose deprivation and modulators on the glycogen content of NSC-34 cells. a Confluent cells were switched to glucose-free DMEM/-FCS and cultured for 12 h (depletion). For repletion, cells were switched to DMEM/25 mM glucose/-FCS for 2 h. b, c Confluent cells were switched to DMEM/5 mM glucose/-FCS for 4 h. Then, modulators were added to a concentration of 100 μM and cells cultured for 30 min. Significant differences are as indicated: *p < 0.05; **p < 0.01; ***p < 0.001