Fig. 7.

siRNA knockdown of GPBB in NSC-34 cells and APC and influence of oxidative stress on the glycogen content after siRNA knockdown. Cells were transfected with either siRNA or NT siRNA and subsequently exposed to oxidative stress. a NSC-34, knockdown without oxidative stress. b NSC-34, knockdown with subsequent exposure to oxidative stress. c APC, knockdown with subsequent exposure to oxidative stress. d Western blot analysis for demonstration of knockdown in NSC-34 cells and APC. For NSC-34, 30 μg of protein were applied in each lane; for APC, 6 μg of protein were applied. Significant differences are as indicated: *p < 0.05; ***p < 0.001