Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 29;12(5):422. doi: 10.1038/s41419-021-03703-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A The immunoreactivity of CD163 was assessed by IHC analysis. B Kaplan–Meier curves for overall survival of GC patients. C The correlation between CD163 and POU1F1 in GC was determined by Pearson correlation analysis. D Cell surface CD14, CD11b, F4/80, and CD11c expression were analyzed by flow cytometry. E Cell viability of macrophages was monitored by CCK-8 assay. F Cell migration of macrophages was determined by transwell migration assay. The mRNA (G) and protein (H) levels of CD163 and CD206 were detected by qRT-PCR and western blot, respectively. CD11b served as an internal control. (I) The immunoreactivity of CD31 was assessed by IHC analysis. J The correlations between CD31 and CD163 in GC were determined by Pearson correlation analysis. K Cell viability of HUVECs was monitored by CCK-8 assay. L In vitro angiogenesis was monitored by tube formation assay. M The protein level of VEGF was determined by western blot. GAPDH served as a loading control. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. *P < 0.05, **P < 0.01.