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. 2021 Apr 29;12:2469. doi: 10.1038/s41467-021-22729-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

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Fig. 3 — a Design of the ZIKV ST experiment. b ST rate (%) of the 2 × scr and miRNA-targeted ZIKVs. c Infection rate (%) of indicated viruses in AG129 female mice following intravaginal inoculation with the dose of 10⁵ pfu of each virus. Difference in the ST rates (b) and in the intravaginal infection rates (c) between 2 × scr and miRNA-targeted viruses was compared using two-tailed Fisher’s exact test followed by Bonferroni correction for multiple comparisons (ns, p > 0.999). d Stability of miRNA targets or scr sequences in ZIKV genomes recovered from the serum of female mice following male-to-female sexual virus transmission. e–h Mean titer ± standard deviation (SD) at 17 dpi and miRNA target stability of ZIKVs recovered from the testis (e), epididymis (f), vas deferens (VasD) (g), and SV/P (h) of male mice participating in the mating experiment. Reproductive organs (number is shown in each panel) were analyzed only for those males whose female mating partners developed detectable ZIKV viremia. (Note: vas deferens and SV/P were not collected from 4 out of 9 male mice infected with 2 × 202(T) and from 1 out of 3 mice infected with 2 × 202/141(T) viruses). The dashed lines indicate the limit of virus detection: 1.7 log₁₀(pfu/g) for testis and SV/P (e, h) and 0.7 log₁₀(pfu/mouse) for epididymis and vas deferens (f, g). i Sequence comparison of the 2 × 202/141(T) virus recovered from female serum and from different organs within the MRS of the corresponding mating male partner. Esc escape mutant, @ virus titer in the testis and SV/P is expressed as log₁₀(pfu/g), & virus titer in the epididymis and vas deferens is expressed as log₁₀(pfu/mouse). NA—virus titer or target stability was not assessed. Differences between the titers of 2 × scr and each of the miRNA-targeted viruses in the mouse organs were compared using one-way ANOVA with multiple comparison adjustment (Dunnett’s test). **p < 0.01; ***p < 0.001; ****p < 0.0001. p values (from left to right): e ns, p = 0.3338. f ns, p = 0.5073; ns, p = 0.8552. g ns, p = 0.8181, h ns, p = 0.1116. ND—p-value is not determined.