Fig. 4. Genome targeting for mir-141 restricts ZIKV shedding into the semen between 7 and 11 dpi.
a Experimental design. Male AG129 mice were infected IP with 106 pfu of 2 × scr or 2 × 141(T) virus, and between 7 and 11 dpi mice were allowed to mate with two CD-1 females in the individual cages. b Mean virus titer ± SD in the ejaculate of AG129 mice that was deposited into FRS of CD-1 mice. Each dot represents an average ZIKV titer in the FRS of CD-1 females that developed vaginal plug (one or two females per cage) after mating with ZIKV-infected male partner. The dashed line indicates the limit of virus detection: 1.0 log10(pfu/g of FRS). Differences between viral titers in the FRS were compared using two-tailed Mann–Whitney test.