Fig. 6. Tracing individual ZIKV genomes during male to female ST of ZIKV-lib/n11 viruses.

Four male AG129 mice infected with ZIKV-lib/n11 deposited ZIKV+ ejaculate into FRS of CD-1 mice at 10 dpi (see Fig. 5b). For each mating pair, a top panel shows the frequency of barcodes that together constitute reads₉₀ for the individual male or female organ. Each color represents a unique barcode. Only barcodes with frequencies of >1% in the FRS are shown as blue or red colors. Sequences and frequencies of these barcodes are presented in the middle panels. “Yes” or “No” denote whether the barcodes were among the reads₉₀ for the given organ. Bottom panels for each mating pair show the titers of ZIKV-lib/n11 viruses in mouse organs collected at 10 dpi. Virus titers in the FRS (ejaculate), testis, SV/P, and brain of males are expressed as log₁₀(pfu/g). Virus titers in the epididymis and vas deferens are expressed as log₁₀(pfu/mouse).