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. 2021 Apr 29;11:9249. doi: 10.1038/s41598-021-88693-8

Figure 3.

Figure 3

NALL restores autophagy flux after TBI. (a) Western blot of cortical tissue lysates from sham and TBI mice fed with NALL or vehicle for autophagosomal marker LC3 and autophagic cargo adaptor proteins p62/SQSTM1 and (b,c) corresponding densitometric analysis. Data are presented as mean ± SEM. n = 5 (only 3/group included for representative images for western blot analysis), *p < 0.05 (Two-way ANOVA with Bonferroni posttests). (d) IF staining for p62/SQSTM1 on frozen sections prepared from mouse cortical brain sections at 1 day after TBI. (e) Quantification of image data from d. Data are presented as mean ± SEM. n = 5/group, *p < 0.05 (Two-way ANOVA with Sidak posttests).