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. Author manuscript; available in PMC: 2021 Sep 8.
Published in final edited form as: Nat Chem Biol. 2021 Mar 8;17(5):593–600. doi: 10.1038/s41589-021-00757-y

Extended Data Fig. 10. Modulation of O-GlcNAc modification level with nUbc-splitOGA on c-Fos-Ubc in comparison to OGT inhibition.

Extended Data Fig. 10

Immunoblotting analysis of protein expression and O-GlcNAcylation status of c-Fos-Ubc and endogenous c-Jun under the indicated treatments corresponding to Fig. 5d, e. by either enrichment against EPEA-tag (a) or chemoenzymatic labeling followed with Biotin-IP (b). Endogenous c-Jun shows negligible changes on O-GlcNAcylation status with the co-expression of nUbc-splitOGA but shows reduced O-GlcNAc modification upon OGT inhibition with OSMI-4b. No detectable endogenous c-Fos was observed in HEK 293T cells. The data in a and b are representative of two biological replicates.